Research on Immunoglobulin Detection using graphene platform is published in Advanced Functional Materials
Our latest research on highly selective electrochemical IgG biomarker detection base on Laser-ablated graphene platform has been accepted in the Advanced Functional Materials. The research is accomplished by Mr. Sharat Chandra Barman followed by Md. Abu Zahed, Md. Sharifuzzaman, Seokgyu Ko, Hyosang Yoon, Joong San Nah and Xing Xuan under the direct supervision of Professor Jae Y. Park.
In this research, a facile, cost-effective, disposable, and highly selective electrochemical immunosensing platform is developed based on cationic polyelectrolyte polyallylamine (PAAMI) anchored laser-ablated graphene (LAG). Here, for the first time, PAAMI is introduced to stabilize LAG flakes, while retaining the intrinsic thermal and electronic properties of the substrate by noncovalent π–π interaction and electrostatic physical absorption. The sensing platform offers a suitable number of anchoring sites for the immobilized antibodies by providing - NH2 functional groups. The proper grafting of PAAMI is confirmed through X-ray photoelectron spectroscopy and Raman spectroscopy. The immunosensing platform is applied to detect immuno-globulin (IgG) biomarkers as a proof of concept. Under optimized conditions, the sensing platform exhibits a linear range of 0.012–15 and 15–352 ng mL−1 with a limit of detection of 6 pg mL−1 for IgG detection with high selectivity. Based on the analysis, the developed immunosensing platform can be used for point-of-care detection of IgG in clinical diagnostic centers. Furthermore, the developed strategy is well suited for the detection of other cancer biomarkers after immobilizing the relevant antibodies.
The research was supported by the Bio & Medical Technology Development Program of the NRF grant funded by the Korean government (MSIT) (NRF-2017M3A9F1031270). Furthermore, the authors are grateful to the members of the MiNDaP (Micro/Nano Devices and Packaging Lab.) group for their technical support and discussion. Full article is available the link below:
