This work reports a novel flexible triboelectric nanogenerator (TENG) based on the conductive nylon membrane (NM). Multiwall carbon nanotubes were directly composited with the poly(3,4-ethylenedioxythiophene)–poly(styrenesulfonate) solution and deposited on one side of the NM surface through vacuum filtration method. The sheet resistance of the fabricated composite material was found as 24 Ω/square and the charged output voltage of 10 µF capacitor was 7.9 V. The NM-based TENG showed robust output power when NM contacted with friction layer of polytetrafluoroethylene (PTFE). The friction layer was consisted of adhesive conductive fabric attached with PTFE. Due to its well flexibility, simple process of fabrication and high output performance, the generator has much potential for powering up portable electronics.